Vol. 10, Special Issue 11 (2021)

Author(s):
Kamatchi Devi S and Dr. S Shanmugasundaram

Abstract:
The main objective of this work is to study the electrochemical oxidation using buffer electrolytes for detecting tetracycline in honey. The three electrode system was used to conduct the optimization studies of different buffers (citric acid (CA), sulphuric acid (SA) and hydrochloric acid (HA)) at pH ranging from 2 to 6 using CV. SA buffer at pH 4 provided the maximum peak current of 1.529 mA at 1.399 V and was optimized for further studies. The detection of TCHC at concentration ranging from 10 µl/l to 100 µl/l was studied by both CV and DPV with a screen printed electrode. From the calibration curve of CV and DPV at different concentration of TCHC, it was observed that DPV shown a better detection with correlation coefficient of 0.9709. Under the optimized conditions, the response of DPV was used to detect the TCHC concentration in honey. The limit of detection was found to be 0.77 µl/l with a percentage recovery ranging between 74 and 116.7%.