Vol. 10, Special Issue 11 (2021)

Author(s):
Anu Malik, Akhil Gupta, Swati Dahiya, Vaishali Sehrawat and Sangeeta Das

Abstract:
With the expanding importance and prevalence of protein therapeutics, recombinant proteins offer different applications in diverse fields including therapeutics and research. For the efficient production of target proteins, various expression systems are available in the market including prokaryotic, eukaryotic and mammalian systems. Pichia pastoris (P. pastoris) is the most widely used novel expression cassettes with the scope of genetic engineering and cell modifications in secretion pathways. P. pastoris can be considered as a distinguished production system for its growth to very high cell densities, for the available strong and tightly regulated promoters, and for the options to produce gram amounts of recombinant protein per litre of culture both intracellularly and in secretory fashion. Thus, making P. pastoris a successful expression system for recombinant protein production with due scope of improvements for future discoveries. We review the characteristic features of P. pastoris as eukaryotic host system for the expression of recombinant proteins and advantages over other expression systems.