Vol. 12, Issue 1 (2023)

Author(s):
S Aasritha, M Lakshman, S Soujanya, M Usharani, M Architha, Y Ravikumar and B Swathi

Abstract:
Present study was aimed to evaluate the individual and combined toxic effects of imidacloprid (IMI) and chlorpyrifos (CPF) on liver of male Wistar rats. Atotal of 24 adult male Wistar albino rats were procured and divided into 4 groups consisting of 6 rats in each. The group1served as control, group 2 rats treated with IMI (@ 10 mg/kg b.wt, once daily orally), group 3 rats were treated with CPF (@ 7.5 mg/kg b.wt, once daily orally) and group 4 administered with IMI (@ 10 mg/kg b.wt, once daily orally) + CPF (@ 7.5 mg/kgb.wt, once daily orally) for 28 days. Rats were sacrificed on 29^th day of experiment. Biochemical parameters were estimated from serum. Tissue samples of liver were collected for histopathology and for analysis of tissue antioxidant profiles. The present study revealed significant (p<0.05) reduction AST and ALT in groups 2, 3 and 4 when compared to group1. The tissue oxidative stress parameters revealed a significant (p<0.05) increase of TBARS/MDA, decrease in GSH and SOD activity in groups 2, 3 and 4 compared to group 1 on 29^th day of the experiment. Liver sections on 29^th day in group 2 showed cloudy swelling, focal necrosis, disruption of hepatic cords and mild sinusoidal congestion. Sections from group 3 revealed congestion of central vein and portal vein, dilated sinusoids, inflammatory cell infiltration in periportal area while, group 4 rats revealed more severe lesions like centrilobular necrosis, diffuse vacuolar degeration of hepatocytes, few pyknotic nuclei, karyorrhexis and karyolysis than in group 2 and 3 rats on 29^th day of experiment.