Vol. 12, Issue 1 (2023)

Author(s):
Prashanth Anur Gopal, HD Narayanaswamy, ML Sathyanarayana, Suguna Rao, D Rathnamma and Divakar Hemadri

Abstract:
The main focus of this study was on respiratory tract pathology and molecular diagnosis of Newcastle disease virus (NDV) in chickens (n=783) from 50 poultry farms in Karnataka during May 2017 to May 2018. New Castle disease (ND) was confirmed in 8 broiler farms, 4 layer farms and 12 indigenous farm birds. Main clinical signs recorded were anorexia depression greenish white diarrhoea, ruffled feathers with droopy wings along with nasal discharge and cough. Grossly, congestion of respiratory tracts with pneumonic changes in lungs, laryngitis, tracheitis, airsacculitis and mucous exudate in nasal passage were seen. Histopathology revealed nasal mucosal thickening with glandular hyperplasia, congestion/ hyperemia, edema and mononuclear cell infiltration in mucosal and submucosal blood vessels along with respiratory epithelial atrophy. Phylogenetic tree construction analysis revealed 100% similarity between field and many published strains as far as 356 bp F gene segment was concerned.