Vol. 10, Issue 3 (2021)

Author(s):
Akshay R Patil, Bhavana A and Deepak

Abstract:
Cyclocarya paliurus has been used commonly to treat diabetes in China. However, the effective components and the effect of plant origin remain unclear. In this study, C. paliurus leaves with different chemical compositions were selected from five geographical locations, and their effects on streptozotocin (STZ)-induced diabetic mice were evaluated with both ethanol and aqueous extracts. Glucose levels, lipid levels, and biomarkers of liver and kidney function were measured. Results showed that C. paliurus extracts with better antihyperglycemic effects were characterized by higher contents of total flavonoids, especially quercetin-3-O-glucuronide and kaempferol-3-O-glucuronide. Furthermore, significantly negative correlations were found between triterpenoids contents and lipid levels. The anti-hyperlidemic effect of flavone-rich B. chinensis leaf extract (HTP) in ICR mice fed a high-fat diet. Treatment with HTP significantly decreased peri-epididymal fat weight (p< 0.01 and p< 0.05 for 200 and 100 mg/kg, respectively), lowered serum and hepatic lipid, and decreased glucose area under curve (AUC) in oral glucose tolerance test (p< 0.01 for 200 mg/kg). Western blot and ELISA analysis showed that administration of HTP (200 mg/kg) significantly increased AMPK (p< 0.05) phosphorylation and PPARα expression in liver (p< 0.05).