CRISPR/Cas9: A versatile tool for genome editing in crop improvement
BS Thorat, SG Bhave, SG Shinde, RL Kunkerkar, BD Waghmode, SS Desai, VV Dalvi, UB Pethe and SV Sawardekar
In a recent era of modernity, a lot of trends have been set in Science and Technology. Despite of vast discoveries always some gaps exists. To bridge up them new trends are set which are commonly known as emerging trends. Although a number of emerging trends have been established but they are specific in their nature to fix the emerging or previous issues. Now a days, in the biological sciences, CRISPR/Cas9 system an emerging approach has been agreed to accomplish the task associated with genome editing (DNA). CRISPR/Cas9 (clustered regularly interspaced short palindromic repeat)-Cas9 is a multipurpose technology for genetic engineering that relies on the complementarity of the guide RNA (gRNA) to a specific sequence and the Cas9 endonuclease activity. It has broadened the agricultural research area, bringing in new opportunities to develop novel plant varieties with deletion of detrimental traits or addition of significant characters. This RNA guided genome editing technology is turning out to be a ground-breaking innovation in distinct branches of plant biology. CRISPR technology is constantly advancing including options for various genetic manipulations like generating knockouts; making precise modifications, multiplex genome engineering and activation and repression of target genes. This system used for improvement of plant nutrition’s, enhancement of plant disease resistance and production of drought tolerant plants. This revolutionary system is easy to handle, eco-friendly and easily executable in every biological field including agriculture, health, virology and genetic disorders.
How to cite this article:
BS Thorat, SG Bhave, SG Shinde, RL Kunkerkar, BD Waghmode, SS Desai, VV Dalvi, UB Pethe, SV Sawardekar. CRISPR/Cas9: A versatile tool for genome editing in crop improvement. Pharma Innovation 2021;10(11):105-120.