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Vol. 8, Issue 7 (2019)

Molecular characterization and structural analysis of fish protein hydrolysate from bycatch Upeneus teniopterus (Cuvier, 1829)

Author(s):
Akhila Narikimelli, S Vincent, Prathapavarma Digala and K Manoj Dhanraj
Abstract:
Fish is usually regarded as a quality protein sources for human and animal consumption, rich in essential amino acids and good for digestibility. Fish by-product also contains the same valuable protein as the fish muscles. Recovery of proteins presents in the by-products and using these as functional ingredients, such as bioactive peptides is a very exciting and promising alternative. By using enzyme technology, it may be possible to produce a broad spectrum of food ingredients for wide range of applications. In the present study molecular characterization and structural analysis of fish protein hydrolysate from by catch fish Upeneus teniopterus were investigated. The electrophoretic techniques (SDS – PAGE) are promising tools for identifying protein profile in fish protein hydrolysate from by catch fishes. The samples Upeneus teniopterus control (UTC) and Upeneus teniopterus control - lyophilized (UTCL) had higher molecular weight bands; whereas in Upeneus teniopterus hydrolysate (UTH) and Upeneus teniopterus hydrolysate – lyophilized (UTHL) showed low molecular weight bands as acid aided in breakdown of protein into small peptides. The HR-SEM analysis was conducted to observe the microstructure of UTCL and UTHL. The microstructure of samples was obtained at different magnifications like 500x, 1000x and 1200x. HR-SEM analysis of UTCL and UTHL revealed different morphological structures due to degree of hydrolysis. The changes in functional properties of native proteins are related to produce by enzymatic hydrolysis, which are mainly characterized by a lower molecular mass, exposure of hydrophobic groups and by an increased number of ionic groups. Fish protein hydrolysate has a broad spectrum of applications ranging from high-value peptones to food ingredients with special functional properties.
Pages: 99-104  |  943 Views  263 Downloads


The Pharma Innovation Journal
How to cite this article:
Akhila Narikimelli, S Vincent, Prathapavarma Digala, K Manoj Dhanraj. Molecular characterization and structural analysis of fish protein hydrolysate from bycatch <em>Upeneus teniopterus</em> (Cuvier, 1829). Pharma Innovation 2019;8(7):99-104.

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