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Vol. 8, Issue 12 (2019)

Standardization of high performance thin layer chromatographic (HPTLC) technique for detecting antibiotic residues in carp (Catla catla) and Gold fish (Carassius auratus)

Author(s):
Akshaya Lakshminarayanan, Ghadevaru Sarathchandra, Karal Marx K, Kaliyamurthi V, Uma A and Kandharam P
Abstract:
Aim: The aim of this study was to standardize a High Performance Thin Layer Chromatographic Technique for detection and quantification of the antibiotic Oxytetracycline in Carp and Enrofloxacin and its metabolite Ciprofloxacin in Goldfish.
Methodology: Carp and Goldfish samples were collected from the fish market Chennai. Carp samples were extracted for the antibiotic Oxytetracycline, and Goldfish samples were extracted for the antibiotic Enrofloxacin and Ciprofloxacin and detected using a CAMAG HPTLC Scanner-3.
Results: The limit of detection and limit of quantification were ascertained for the antibiotics by High Performance Thin Layer Chromatography. The recovery percentage recorded is above 85% for the antibiotics. Linearity, standard peak and Rf value for the antibiotics were also obtained.
Interpretation: This study explains that High Performance Thin Layer Chromatography has a unique advantage of high throughput, economical and a simple analytical tool that can be used for the analysis of antibiotic residues.
Pages: 394-400  |  292 Views  67 Downloads


The Pharma Innovation Journal
How to cite this article:
Akshaya Lakshminarayanan, Ghadevaru Sarathchandra, Karal Marx K, Kaliyamurthi V, Uma A, Kandharam P. Standardization of high performance thin layer chromatographic (HPTLC) technique for detecting antibiotic residues in carp (<em>Catla catla</em>) and Gold fish (<em>Carassius auratus</em>). Pharma Innovation 2019;8(12):394-400.

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