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Vol. 7, Issue 8 (2018)

Vascular remodelling increases nitric oxide mediated vasorelaxation of uterine artery in pregnant goat (Capra hircus)

Author(s):
Harithalakshmi Jandhyam, Soumya Ranjan Rout and Subas Chandra Parija
Abstract:
The present study investigates the role of NO in vasorelaxation of middle uterine artery obtained from non-pregnant (NP) and pregnant (P) Capra hircus (Ch). Uterine artery rings (1.5-2 mm) were mounted in a four chambered automatic organ bath containing 20 ml MKHS, maintained at pH 7.4. Following 1hr equilibration, Phenylephrine (PE) was added to bath in cumulative dose (1ηM-10μM) or in single dose (1μM) to induce contraction. ACh (10ηM-10μM) was added cumulatively to the bath either in absence or presence of L-NAME or Indomethacin or combination of L-NAME and Indomethacin or SNP (10ηM-10μM) to relax PE-precontracted rings. Isometric contraction was recorded using highly sensitive force transducer connected to power Lab (8/16) data acquisition system. The maximal contraction (Emax) obtained from CRC of PE elicited in MUA of P Ch ( 2.19± 0.26g) was significantly greater than that of NP Ch (1.61±0.01g). PE-induced sustained contraction (100%) was reduced to 67.77%, 57.88% by ACh and 47.28%, 31.30% by SNP in MUA of NP and P Ch, respectively. Endothelium removal in MUA rings almost abolished ACh –contraction in NP and P Ch. In presence of L-NAME or Indomethacin or L-NAME and Indomethacin, ACh-induced contraction was augmented to 90.54%, 74.74%, 91.43% in NP and 87.38%, 88.21%, 85.82% in P Ch, respectively. In conclusion, vascular remodeling of uterine artery in pregnant goat (i) increased vascular resistance is due to increased sensitivity of α1 adrenoceptor, (ii) augmented vasorelaxation to ACh due to endothelial activation of eNOS-NO-cGMP /COX-NO-cAMP pathways. We observed for the first time that vascular remodelling in pregnancy augmented the contraction and relaxation of uterine artery of goat is due to increased sensitivity of α1-adrenergic receptor and EDRF.
Pages: 205-211  |  810 Views  76 Downloads


The Pharma Innovation Journal
How to cite this article:
Harithalakshmi Jandhyam, Soumya Ranjan Rout, Subas Chandra Parija. Vascular remodelling increases nitric oxide mediated vasorelaxation of uterine artery in pregnant goat (<em>Capra hircus</em>). Pharma Innovation 2018;7(8):205-211.

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