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Vol. 7, Issue 5 (2018)

In vivo assessment of lipid peroxidation and antioxidant profile in aqueous and ethanolic extract ointment of Salix acmophylla leaves in rabbits for wound healing improvement on excisional full thickness skin wounds

Author(s):
Qumaila Sakeena, Md. Moin Ansari, Sadaf Sakeena, Shawkeen Muzamil and DM Makhdoomi
Abstract:
The present study has been designed to estimate and evaluate the concentration of oxidant i.e. lipid peroxidation (nmol of melonialdehyde/g of tissue) coloured adduct (melonialdehyde) was read at 532 nm on spectrophotometer and antioxidants i.e. catalase (IU/mg of skin tissue) in the skin tissue using spectrophotometer at 240nm and levels of zinc (µg/ml of serum) and copper (µg/ml of serum) using atomic absorption spectroscopy method at 213.9nm and 324.8nm respectively in rabbits after creation of excisional full thickness skin wound and post treatment with the 5% ethanolic and 5% aqueous extract ointment group (6 animals each) and comparing the results with the control group (6 animals). The Lowest concentration of lipid peroxidation (melonialdehyde) and highest concentration of catalase enzyme and trace metals was seen in 5% ethanolic extract ointment treated group with fastest wound closure in just 14.50± 0.42 days followed by 5% aqueous extract ointment treated group in in 17.16±0.30 compared to control group 20.16±0.30. These in vivo results strongly suggest the significant and beneficial effects of Salix acmophylla leaves ointment for the improvement of wound healing and can be exploited to accelerate excision wound healing.
Pages: 492-500  |  629 Views  119 Downloads


The Pharma Innovation Journal
How to cite this article:
Qumaila Sakeena, Md. Moin Ansari, Sadaf Sakeena, Shawkeen Muzamil, DM Makhdoomi. In vivo assessment of lipid peroxidation and antioxidant profile in aqueous and ethanolic extract ointment of Salix acmophylla leaves in rabbits for wound healing improvement on excisional full thickness skin wounds. Pharma Innovation 2018;7(5):492-500.

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