Author(s):
V Ravikanth, M Lakshman, D Madhuri and B Kalakumar

Abstract:
The present study was designed to know the histopathological and ultrastructural alteration due to imidacloprid and spinosad (neonicotinoid and bacterial insecticide respectively) and their amelioration with vitamin E and silymarin in male Cobb broiler chicken. The experiment was carried out for 28 days. Histopathologically, group 2 liver section revealed congestion of central vein. In group 3 birds, the lesions found in liver sections were varied degrees of degeneration in hepatocytes. Histopathological changes of liver in group 4 were severe congestion of central vein and sinusoidal spaces. The microscopic changes in group 5 liver were mild vacuolar degeneration and group 6 liver sections revealed moderate to severe vacuolar degeneration. Ultrastructurally, group 2 liver revealed altered shape and size of nucleus and mitochondria. Group 3 liver section revealed mild hypertrophy of Kupffer cells and condensed mitochondria. Group 4 liver revealed swollen hepatocyte, disrupted and discontinuous rough endoplasmic reticulum. Group 5 liver was characterized by vacuolation in cytoplasm and variation in shape and size of nucleus. Group 6 liver showed mild to moderate vacuolar degeneration in cytoplasm.