Vol. 12, Special Issue 9 (2023)

Author(s):
Poloju Deepa, Koppu Vasavi, Kavitha Madineni, Mudasir M Rather, Bhavani Puvvala, Shilpa Balaji and Chandana MS

Abstract:
Bovine tuberculosis (bTB), caused by members of the Mycobacterium tuberculosis complex (MTBC), poses a significant threat to both cattle and human health. This research employs a molecular approach to detect and identify MTBC isolates from bovine lymph nodes. A combination of culture-based isolation, biochemical tests, and polymerase chain reaction (PCR) analysis was used to enhance sensitivity and accuracy. The study identified MTBC isolates in lymph nodes of infected cattle. The findings underscore the importance of molecular methods in the surveillance and control of bTB.