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Vol. 12, Special Issue 9 (2023)

Study on association of sirtuin 1 and 3 gene expression with metabolic profile of indigenous pigs and piglet

Author(s):
Shubham Gawade, Sameer Jadhav, Gokul Sonawane, Uma Tumlam and Abhijit Barate
Abstract:
Poor metabolic capacity affects overall health status of piglets and it is mainly associated with various piglet associated health problems. Several studies have cited the role of histone deacetylating enzymes called sirtuins, mainly sirtuin 1 and 3 in regulating nutrient sensing pathways, caloric restriction response and thus regulating overall cellular metabolism of animals. The research was conducted to study the expression level of sirtuin 1 and 3 genes in blood samples in indigenous piglets (N=8) relative to adult pigs (N=8) using de novo custom synthesized primers of sus scrofa species. Beside this serum metabolic profile was tested and compared between piglets and adult indigenous pigs. The data of the gene expression study were correlated with serum metabolic profile to assess the significant correlation of metabolic health status of pigs with sirtuin gene expression. The expression of sirtuin1 gene was found significantly lower (p< 0.01) and expression of sirtuin 3 gene was found significantly higher (p< 0.01) in piglets relative to adult indigenous pigs. Significantly lower values (p< 0.01) of total protein, albumin, globulin, calcium and significantly higher values (p< 0.01) of triglycerides, alkaline phosphatase, phosphorus, lactate dehydrogenases were found in indigenous piglets as compared to adult pigs. The values of total protein, albumin was found positively correlated (p> 0.05) and triglycerides, phosphorus and alkaline phosphatase (p> 0.05) were found negatively correlated with sirtuin I fold expression level. Values of glucose (p> 0.05), BUN, triglyceride, ALP and lactate dehydrogenase (p> 0.01) were found positively correlated and values of calcium and albumin (p> 0.05) were found negatively correlated with sirtuin 3 fold expression level.It is concluded that the sirtuin 1 and 3 expression levels varies in two age groups of indigenous pigs and are also associated with overall metabolic status of pigs.
Pages: 1851-1856  |  76 Views  41 Downloads
How to cite this article:
Shubham Gawade, Sameer Jadhav, Gokul Sonawane, Uma Tumlam and Abhijit Barate. Study on association of sirtuin 1 and 3 gene expression with metabolic profile of indigenous pigs and piglet. The Pharma Innovation Journal. 2023; 12(9S): 1851-1856.
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