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Vol. 12, Special Issue 9 (2023)

Characterization of indigenous Bacillus thuringiensis (Bt) isolates and screening for lepidopteran toxic insecticidal genes

Author(s):
Thilagarajan R, N Balakrishnan, G Rajadurai, Sheela Venugopal, R Raghu and E Kokiladevi
Abstract:
Bacillus thuringiensis (Bt) is a widely distributed Gram-positive bacterium known for its ability to produce insecticidal toxins with high specificity for certain insect pests. In this study, 15 indigenous Bt isolates were characterized and screened for lepidopteran toxic insecticidal genes through PCR. The colour, surface, shape, elevation and margin of the isolates were studied. Diversified crystal shapes were observed among the isolates tested. PCR analysis confirmed the presence of cry1, cry2 and vip3A genes in the isolates, either individually or in combination, while none of the isolates possessed cry9 gene.
Pages: 1691-1694  |  126 Views  91 Downloads
How to cite this article:
Thilagarajan R, N Balakrishnan, G Rajadurai, Sheela Venugopal, R Raghu and E Kokiladevi. Characterization of indigenous Bacillus thuringiensis (Bt) isolates and screening for lepidopteran toxic insecticidal genes. The Pharma Innovation Journal. 2023; 12(9S): 1691-1694.
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