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Vol. 12, Special Issue 9 (2023)

Studies on genetic variability in sheep prevalent at Nagarjuna Sagar region of Telangana using microsatellite markers

Author(s):
A Suvarna, D Anil Pavan Kumar, P Jaya Laxmi and RMV Prasad
Abstract:
Using ten microsatellite markers, the genetic diversity of the local sheep that are common in Telangana's Nagarjuna Sagar region were genotyped. Ten microsatellite markers and a total of 82 alleles were examined. OarAE129 and OarCP38 had the fewest alleles (6 each), whereas MAF214 had the most alleles (11), with a mean of 8.2 alleles. With a mean value of 7.221, the effective number of alleles ranged from 4.810 (OarAE129) to 9.970 (MAF214). While the allele frequencies ranged from a minimum of 0.050 (BM8125, INRA063, MAF214) to a maximum of 0.315 (OarAE129), the allele sizes varied from a minimum of 112bp in OarCP38 to a maximum of 264bp in MAF214.The mean heterozygosity that was observed and predicted, respectively, was 0.104 and 0.855. The mean inbreeding coefficient across the board was 0.878. The FIS values were between 0.832 to 0.941 (OarCP34 to BM8125). The study's inbreeding estimations were all positive, indicating a severe shortage of heterozygotes. The PIC values for the ten microsatellite loci varied from 0.762 (OarAE129) to 0.890 (MAF214) and all ten loci (100%) were determined to be highly polymorphic. It was discovered that the mean PIC value across all ten loci was 0.836. The chi-square test showed that all 10 loci were significantly deviating from the Hardy-Weinberg equilibrium adding to our understanding of the genetic makeup of this indigenous sheep. High genetic variability within the breed was found by the results.
Pages: 964-967  |  62 Views  31 Downloads
How to cite this article:
A Suvarna, D Anil Pavan Kumar, P Jaya Laxmi and RMV Prasad. Studies on genetic variability in sheep prevalent at Nagarjuna Sagar region of Telangana using microsatellite markers. The Pharma Innovation Journal. 2023; 12(9S): 964-967.
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