Vol. 12, Special Issue 9 (2023)

Author(s):
Gurusrinath Reddy M, Vijayalakshmi S, Raniprameela D and Sailaja N

Abstract:
In the present study a total of 148 lung tissues from sheep with gross pathological lesions like congestion, consolidation and hepatization were collected in a micro centrifuge tube containing PPLO broth during postmortem examination and slaughter. Out of 148 lung tissues, 52 samples were found to be positive for genus Mycoplasma yielding 280 bp product. Out of 52 Mycoplasma positive samples 53.84% (28/52) lung tissues were found to be positive for Mycoplasma ovipneumoniae. Cultural and biochemical isolation studies of 28 lung swabs from Mycoplasma ovipneumoniae positive samples on selective media viz., blood agar, brain heart infusion agar, Macconkey agar, mannitol salt agar revealed Pasteurella multocida 7/28(25%), Mannheimia haemolytica 5/28(17.85%), E. coli 9/28(32.14%), Klebsiella 6/28(21.4%), S. aureus 10/28(35.71%) and Streptococcus spp 7/28(25%).