Vol. 12, Special Issue 9 (2023)

Author(s):
Gagan, Renukaradhya GJ, Sahadev A, Naveenkumar S, Kalmath GP, Suchitra BR, Ningaraju K and Rashmi S

Abstract:
The present study was carried out with the objectives to evaluate seminal attributes and to study the motion kinematics of Tharparkar breeding bull semen using computer assisted semen analyzer (CASA) outside its breeding tract. Semen was collected bi-weekly from three Tharparkar bulls for 10 weeks. The spermiogram revealed, the colour variation among the bulls was from milky to creamy white, consistency (0-4 scale) 2.57 ± 0.07, ejaculate volume 3.97 ± 0.16 mL, sperm concentration 1303 ± 51.29 million / mL, mass activity (0-5 scale) 3.48 ± 0.07, initial motility 81.50 ± 0.68%, sperm viability 86.72 ± 0.46%, total sperm abnormality 14.13 ± 0.20%, acrosomal integrity 92.82 ± 0.24% and HOST 86.78 ± 0.43 percent (80.00 to 92.00). The motion kinematics (μm/s) of neat semen was: VCL (Curvilinear velocity) 132.00 ± 1.74, VSL (Straight-line velocity) 74.21 ± 1.28 and VAP (Average path velocity) 87.14 ± 1.45. The post thawed semen characters revealed motility of 61.50 ± 0.49%, HOST 59.87 ± 0.60% and the motion kinematics (μm/s) was: VCL 111.10 ± 1.99, VSL 65.61 ± 1.28 and VAP 79.48 ± 1.48. Significant (p<0.01) reduction in seminal characters of post thawed semen was noticed. It was concluded that seminal attributes of Tharparkar bulls fall within the range established for other Zebu cattle and CASA found to be more efficient and precise means of qualitative evaluation of motion kinematics.