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- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
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Vol. 12, Special Issue 9 (2023)
Prevalence and molecular characterisation of extended spectrum β-Lactamase resistant determinants in Salmonella spp. isolated from Swine, Tirupati district, Andhra Pradesh
Author(s):
Someswari U, Sreedevi B, Vinod Kumar N and Jagadeesh Babu A
Someswari U, Sreedevi B, Vinod Kumar N and Jagadeesh Babu A
Abstract:
Pigs and Pork serve as an important reservoir of Salmonella spp., which is still the second-greatest cause of food-associated hospitalizations globally. Hence, surveillance studies on antimicrobial resistance in Salmonella are essential. The aim of the present study is to determine the prevalence, antibiotic susceptibility profile and molecular characterization to detect resistant determinants in Salmonella spp. isolated from swine in Tirupati, Andhra Pradesh. Salmonella was identified using cultural and biochemical characteristics and positive isolates were assessed for antibiotic susceptibility and subjected to PCR assay. Collectively, 100 rectal swabs were obtained from pigs maintained on both organized and unorganized farms. We found 164 isolates as Enterobacteriaceae members, of which 28 isolates (17.07%) were Salmonella. Resistance to amoxicillin and colistin was found to be high (100%) followed by ceftriaxone (71.42%), gentamicin (50%), amoxicillin / clavulanic acid (32.14%) and enrofloxacin (10.71%). No isolate was found to be resistant to tetracycline. blaTEM (82.14%) is the most prevalent β – lactamase gene found in the gut microbiota of swine, followed by blaCTX-M group 2 (10.71%), blaCTX-M group 1 (7.14%) and blaSHV (3.57%) genes.
Pigs and Pork serve as an important reservoir of Salmonella spp., which is still the second-greatest cause of food-associated hospitalizations globally. Hence, surveillance studies on antimicrobial resistance in Salmonella are essential. The aim of the present study is to determine the prevalence, antibiotic susceptibility profile and molecular characterization to detect resistant determinants in Salmonella spp. isolated from swine in Tirupati, Andhra Pradesh. Salmonella was identified using cultural and biochemical characteristics and positive isolates were assessed for antibiotic susceptibility and subjected to PCR assay. Collectively, 100 rectal swabs were obtained from pigs maintained on both organized and unorganized farms. We found 164 isolates as Enterobacteriaceae members, of which 28 isolates (17.07%) were Salmonella. Resistance to amoxicillin and colistin was found to be high (100%) followed by ceftriaxone (71.42%), gentamicin (50%), amoxicillin / clavulanic acid (32.14%) and enrofloxacin (10.71%). No isolate was found to be resistant to tetracycline. blaTEM (82.14%) is the most prevalent β – lactamase gene found in the gut microbiota of swine, followed by blaCTX-M group 2 (10.71%), blaCTX-M group 1 (7.14%) and blaSHV (3.57%) genes.
As the colistin is supposed to be the last resort antibiotic in human therapeutic usage. Hence, the validity of colistin resistance is further investigated by E-Test by selecting two isolates that showed colistin resistance by Disc diffusion test. However, the isolates showed sensitivity to colistin with MIC of 1µg/ml of colistin. The isolates showed disparity in colistin resistance when tested by disc diffusion method and E-test. Despite of phenotypic resistance to colistin detected by the Disc Diffusion method, the plasmid-mediated mcr-1 gene could not be found in any isolate.
Pages: 37-48 | 417 Views 146 Downloads
How to cite this article:
Someswari U, Sreedevi B, Vinod Kumar N and Jagadeesh Babu A. Prevalence and molecular characterisation of extended spectrum β-Lactamase resistant determinants in Salmonella spp. isolated from Swine, Tirupati district, Andhra Pradesh. The Pharma Innovation Journal. 2023; 12(9S): 37-48.
Journal code(s)
- E-ISSN Number: 2277-7695
- P-ISSN Number: 2349-8242
- CODEN Code: PIHNBQ
- ZDB-Number: 2663038-2
- IC Journal ID: 7725
Main Menu
Special Issue
Copyright Form
Identifier
The Pharma Innovation Journal
