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Vol. 12, Special Issue 8 (2023)

Differentiation of avian Escherichia coli isolates through RAPD PCR

Author(s):
Sabita Behera, Esha Sinha, Bhavani Puvvala, Manimaran N, Y Sriharsha, Subuhi Khan and Salauddin Qureshi
Abstract:
This study involved thirty E. coli strains isolated from poultry birds from different poultry houses. Blood agar was used for inoculation at first followed by EMB and MacConkey agar for suspected samples only.30 out of 100 samples where bacterial growth was observed were pinned down as E. coli. For the detection of genetic difference between E. coli strains, these strains were typed by random amplification of polymorphic DNA (RAPD). It was followed by amplification of DNA extracted from 30 strains of E. coli by RAPD primer (COL 1) which yielded eight different band profiles. In our study we found great genetic heterogeneity among different E. coli strains. The RAPD analysis, due to its simplicity and rapidity appears to be a highly valuable tool for studying E. coli molecular epidemiology.
Pages: 1203-1205  |  119 Views  74 Downloads
How to cite this article:
Sabita Behera, Esha Sinha, Bhavani Puvvala, Manimaran N, Y Sriharsha, Subuhi Khan and Salauddin Qureshi. Differentiation of avian Escherichia coli isolates through RAPD PCR. The Pharma Innovation Journal. 2023; 12(8S): 1203-1205.
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