The current research work was undertaken to induce estrus synchronization in Kilakarsal ewes, using chitosan Nano conjugated GnRH and to assess its efficacy on the fertility rate of Kilakarsal sheep during the main season under semi-intensive farming conditions by comparing the same with various drug protocols. Ewes in the control group were not given any treatment. The group I ewes were treated with CIDR alone. The group II ewes were treated with CIDR and GnRH. The group III ewes were treated with CIDR and PGF2α. The group IV ewes were treated with CIDR, PGF2α, and GnRH. The group V ewes were treated with CIDR and Nano conjugated GnRH. The group VI ewes were treated with CIDR, PGF2α, and Nano conjugated GnRH. During the main season, the % of ewes that lambed as against the total number of ewes that were mated in each group were 71.42, 81.25, 83.33, 88.23, 94.11, 94.73 and 100.00 in the control and groups I to VI, respectively The highest fecundity % was observed in the groups V (94.73) and VI (100.00) From this research Nanoconjugated GnRH is an economic alternative method that offers reasonable pregnancy rates and it is very safe in Kilakarsal ewes.