Vol. 12, Special Issue 7 (2023)

Author(s):
Akhilesh Pandey, MS Thakur, DS Gonge and Y Pandey

Abstract:
αs2-casein is a primary milk protein. However, there has been little research focused on the effects of αs2- casein variants on milk. The αs2-casein (CSN1S2) play a major protein found in ruminant’s milk, which is encoded by a highly polymorphic CSN1S2 gene present on chromosome 6. Research work was carried out on 50 animals each of Sahiwal and HF Crossbred cow at College of Veterinary Science Jabalpur, Madhya Pradesh, India. The αS2 casein gene (1267 bp) was digested by EcoRV restriction enzyme yielding two genotypes viz., AA (1267 bp) and BB (1267/1150/117 bp) in HF crossbred cattle, while only one genotype (AA) in Sahiwal. The restriction site was absent in Sahiwal resulting in only single compact band of 1267 bp. The tested animals of Sahiwal were found monomorphic at this locus. Association study of different genotypes with milk composition traits revealed that the mean lactose % showed non- significant difference in A1A2 and A2A2 genotype of Sahiwal and HF Crossbred whereas Sahiwal showed non- significantly higher mean value of SNF % compared to HF Crossbred cow milk. The mean milk density (kg/L) was significantly higher in the HF Crossbred milk breed compared to Sahiwal milk. The frequency of A allele was found to be highest as compared to B allele in all the four breeds of cattle under the study. breed of cow. The frequency of A2 allele was found to be highest(1.00) as compared to A1 allele(0.00) in above breeds of cattle under the study.