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Vol. 12, Special Issue 11 (2023)

RNA sequencing: A technique for advancing the field of fish genetics and breeding

Author(s):
Raju Ram, Gowhar Iqbal, Jaspreet Singh, Devarshi Ranjan, Vikas Pathak, Shyam Kumar and Amrita Mohanty
Abstract:
Global gene expression profiling using RNA-seq, enabled by high throughput third-generation sequencing technology, revolutionizes transcriptome exploration. Compared to microarray, RNA-seq has advantages, including a larger dynamic range, accurate transcript quantification, and comprehensive tissue expression coverage. In addition to quantification of the transcript, RNA-seq is used for transcriptome comparison, mapping the both 5' and 3' ends of introns and mRNA, discovery of new exons, research into allele-specific expression, SNP finding, construction of de novo gene models and investigation into non-coding RNA. RNA-seq technology is increasingly utilized in various commercial and ecologically important species-related research for differential gene expression study and SNP discovery. The present review article encapsulates the RNA-seq profiling steps and potential applications in aquaculture.
Pages: 391-394  |  151 Views  96 Downloads
How to cite this article:
Raju Ram, Gowhar Iqbal, Jaspreet Singh, Devarshi Ranjan, Vikas Pathak, Shyam Kumar and Amrita Mohanty. RNA sequencing: A technique for advancing the field of fish genetics and breeding. The Pharma Innovation Journal. 2023; 12(11S): 391-394.
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