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Vol. 12, Special Issue 10 (2023)

Comparison of lateral flow assay and PCR for diagnosis of feline panleukopenia

Author(s):
Manikantaswamy BM, Anil Kumar MC, Soundarya TC, Anjan Kumar KR, Lathamani VS, Chetan Kumar GK, Veena MP, Ramesh PT, Suchitra BR, Shivraj Murag and Sumathi BR
Abstract:
During the period of six months from August 2021 to January 2022, a total of 52 cats with the clinical signs of gastroenteritis presented to Veterinary College Hospital, Hebbal, Bengaluru, were examined for clinical signs suggestive of feline panleukopenia. Feline panleukopenia was diagnosed by PCR and lateral flow assay. Among 52 faecal samples tested by IC strip test and PCR, 23 samples were positive by IC strip test and 38 samples were positive by PCR. Among 38 PCR positive samples, only 23 were found to be positive by IC strip test. All the 14 PCR negative samples were detected as negative by IC strip test. When compared to PCR, the IC strip test had a sensitivity and specificity of 60.52 percent and 100 percent, respectively. This result indicates, IC strip test have high specificity but poor sensitivity when compared to PCR for detection of feline panleukopenia virus in faecal sample.
Pages: 171-174  |  131 Views  72 Downloads
How to cite this article:
Manikantaswamy BM, Anil Kumar MC, Soundarya TC, Anjan Kumar KR, Lathamani VS, Chetan Kumar GK, Veena MP, Ramesh PT, Suchitra BR, Shivraj Murag and Sumathi BR. Comparison of lateral flow assay and PCR for diagnosis of feline panleukopenia. The Pharma Innovation Journal. 2023; 12(10S): 171-174.
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