Vol. 11, Special Issue 9 (2022)

Author(s):
Shivaprasad Ch, Vinoo R, Chatterjee RN, Muralidhar M, Narendranath D, Aswanikumar K, Bhattacharya TK, Sushma G, Kanakachari M and Prasad AR

Abstract:
The present study initiated with an objective of expression profile of Acetyl CoA carboxylase beta (ACACB) gene during pre and post-hatch period in White leghorn IWK line. ACACB gene plays a critical role in fatty acid metabolism thereby potentially regulating meat and egg quality. Temporal expression pattern of the ACACB gene in Quantitative real-time PCR (QPCR) indicated that the gene expresses in all stages in the skeletal muscle of chicken during pre-hatch (embryonic day (ED) 5^th to ED 18^th) and post-hatch (18^th, 22^nd and 40^th week) period. During pre-hatch period highest expression at ED10 (37.86±0.37) and lowest at ED13 (32.15±0.21) was found. However, a significant (p<0.05) differences of gene expression was observed in different embryonic days. Whereas expression levels of ACACB gene during post-hatch period (p<0.005) within group was not significant. The magnitude of ACACB gene expression was highest on 40th week (32.21±0.44) and lowest on 18^th week (29.27±1.16) was observed. The present study suggests that the ACACB gene plays a crucial role in pre and post- hatch period in IWK line.