Vol. 11, Special Issue 9 (2022)

Author(s):
VB Gore, PJ Rathod, AG Vala and D Kumar

Abstract:
Groundnut seed protein SDS-PAGE profiling of spreading varieties were studied with sequential extraction of in defeated powder with water, NaCl, phosphate buffer and alcohol for protein fraction viz., albumin, globulin, glutelin and prolamin respectively. The results of albumin% and globulin% content found to be in range of 16.2 to 20.43% and 72.05-78.5% respectively. The globulin protein fraction content was higher proportion then other three fractions. Glutelin% and prolamin% was found to be very lower in all varieties with the mean of 2.17% and 2.57% respectively. Ten varieties of speeding types were subjected to profiling through SDS-PAGE analysis. The results indicated considerable variation in genetic make-up through total number of bands and MW-Rf values for respective protein fractions. Albumin and globulin had the highest MW-Rf values in bands collectively (20–23), whereas glutelin and prolamin had the lowest MW-Rf values bands with ranged between 6-10. The qualitative and quantitative differences were revealed that seed protein profiles can be the best solution for correcting same protein in varietal identification with MW-RF in SDS PAGE. Correlation matrix between protein fractionation indicated that globulin was negatively correlated with prolamin and glutelin fraction.