Vol. 11, Special Issue 9 (2022)

Author(s):
Nirbhay Kumar Singh, Manoj Kumar Sinha and Vijay Kumar

Abstract:
The present study was designed to study certain histological and histochemical characteristics of gizzard in parent stock of Gramapriya by light microscope. The study was conducted on twelve adult Gramapriya birds of either sex whose weight varied from 1.5 - 2 Kg. All the birds were purchased from Central poultry farm Bihar Animal Sciences University, Patna. Before culling the birds were anaesthetised by giving inhalant anaesthesia and the organs were immediately removed after slaughter. The organs were gently cleaned and fixed in 10% buffered neutral formalin and then processed for various histological and histochemical studies. The histological observations revealed that like other species of birds the Gizzard of Gramapriya consisted of four tunics; tunica mucosa, tunica sumbucosa, tunica musculosa and tunica serosa. The mucosa of gizzard was lined with thick layer of cuticle which consisted of gastric pits. The lamina epithelialis was lined with simple cuboidal epithelium and the lamina propria contained simple tubular glands which were arranged in groups and these glands were lined by simple cuboidal epithelium. The secretion of inner part of the tubular glands was eosinophilic in nature. PAS positive substances were distributed nearly in almost entire region. It was scattered within the lumen of the glands, within the cells lining the surface and crypts. It was also observed within the deep glandular cells. The submucosa consisted of connective tissue formed mainly of collagen fibers. The tunica muscularis was very thick formed from parallel bundles of smooth muscle fibers which were separated by thin layer of connective tissue. The tunica serosa was made up of connective tissue which was covered with mesothelium.