Vol. 11, Special Issue 9 (2022)

Author(s):
Dr. Ajeet Kumar and Dr. Komal

Abstract:
Fis is a transcriptional regulatory protein which regulates the expressions of the capsules in Pasteurella multocida was amplified cloned, sequenced and expressed in prokaryotic expression system. Transformation in E. coli BL21 strain is confirmed by restriction digestion and colony PCR. Cloned Fis gene was also sequenced and BLAST analysis show 100 similarities with Fis gene sequence of other P. multocida. Cloned product was also expressed in E. coli BL21 and characterized by SDS-PAGE and western blotting which can be further used for functional study of Fis gene.