Isolation of microorganisms sensitive to low concentration of pesticides and heavy metals residues
Jasmin M Nadaf and AM Navale
The purpose of this study was to assess the residual pesticide viz, Cypermethrin, Spinosad, Acephate, Carbendazim, as well as Chlorpyriphos, heavy metal viz, Lead, Cadmium, & Cromium, as well as the microbial contamination quality and safety of common marketable fruits and vegetables samples from various local markets in Mumbai. For these study soil samples were taken from the railway site of Malad, Vileparle, Kandivali, Rammandir, Goregaon, as well as Andheri railway stations on Mumbai's western line. Soil samples were gathered and labelled correctly in disposable polythene bags. Ten pesticides and seven heavy metals were used. Ten frequently used pesticides viz; Cypermethrin, Chloropyriphos, Acephate, Spinosad, Captan, Carbendazim, Metalaxyl, Mancozeb, Chlorothalonil, 2-4-D as per European standard concentration. Seven used heavy metals viz; Copper (cu), Lead (Pb), Cadmium (Cd), Chromium (Cr), Nickel (Ni), Arsenic (As), Mercury (Hg) as per Indian standard awasthi (2000). Total 21 isolates were isolated for cypermethrin, 16 for chloropyriphos, 11 for acephate, 16 for spinosad, Captan 22, carbendazim 16, metalaxyl 12, mancozeb 9, chlorothalonil 14 and for 2-4-D 20 isolates were isolated. Among the heavy metals total 30 isolates were isolated for copper, lead 14, cadmium 10, chromium 20, nickel 10, and for arsenic 12 isolates were isolated. These isolates probably belonging to genus Bacillus, Pseudomonas, Paenibacillus, Azotobacter, Azospirillum spp.