Vol. 11, Special Issue 8 (2022)

Author(s):
B Nandini Priya, P Sanjana Reddy, JV Ramana, Jinu Jacob, B Sreekanth and T Nepolean

Abstract:
Simple sequence repeats (SSR) analysis was used in pearl millet in order to assess the degree of polymorphism within and among genotypes and to investigate if this approach was suitable for genetic studies of pearl millet. The genotypes were evaluated using 28 SSR markers that were found to be polymorphic among the 50 SSR markers tested. The genetic distances were calculated using unweighted paired group method with arithmetic averages (UPGMA) method. A total 148 alleles were obtained with an average of 5.37 per locus. The polymorphic information content (PIC) values ranged from 0.74 (PSMP 2078) to 0.93 (PSMP 2214) with an average of 0.83. Comparison of the polymorphic products was generated by Jaccard’s similarity co-efficient and using these values a dendrogram was generated which showed the phylogenetic relationships between the genotypes. The UPGMA analysis indicated a higher similarity between genotype ICMR 07999 and ICMB 98222 and lowest similarity index was observed between 670R and 2B. Analysis of SSR data helped in estimating genetic diversity and distinguish the genetic relationship among genotypes to identify the diverse genotypes which can be further used in hybridization programme.