Vol. 11, Special Issue 8 (2022)

Author(s):
H Jaferin, RP Aravindh Babu, TMA Senthilkumar, G Dhinakar Raj, R Sridhar and A Ramesh

Abstract:
Lumpy skin disease (LSD) in cattle population continues to be a major economic threat among dairy farmers worldwide. Accurate diagnosis of LSD in animals is therefore of utmost importance to reduce the financial burden of farmers. Although there are several methods to detect LSDV in clinical samples, PCR remains the most sought-after method due to its relatively short assay time. In this pilot study we have established a diagnostic PCR method to amplify the complete A33R gene of LSDV genome. This new method is comparable with that of the PCR method prescribed by OIE for diagnosis of LSDV in clinical samples. Furthermore, the complete coding region of the A33R-gene from one of the field isolates was sequenced.