Vol. 11, Special Issue 8 (2022)

Author(s):
Sanjana, Savita Kumari, Anjay, Ajeet Kumar, Pankaj Kumar, Bablu Kumar, P Kaushik and Komal

Abstract:
Uropathogenic Escherichia coli (UPEC) are among the most prevalent pathogens responsible for urinary tract infections (UTIs), accounting for up to 90% of community-acquired and 50% of hospital-acquired UTIs. The ability of UPEC to form biofilms and their role in bladder colonisation play crucial roles in the pathogenesis of UTIs. Bacteriophages are the most potential alternative to antibiotics for clinical usage due to the rise of resistant UPEC strains and a decline in the rate of novel antibiotic development. The lytic bacteriophages were isolated from livestock and poultry sewage samples using E. coli as indicator strain. Phage were characterized based on the morphology using TEM, host range determination, thermal and pH stability. A total of 09 lytic phage were isolated against Uropathogenic E. coli (UPEC) and purified. The morphological characterization revealed that the isolated E. coli phage belong to the family Siphoviridae, Podoviridae and Myoviridae. Phage thermal stability revealed that the phage titre was stable at temperature 37 °C and pH 7.