Vol. 11, Special Issue 8 (2022)

Author(s):
Manisha Sethi, Tushar K Mohanty, Mukesh Bhakat, Nadeem Shah, Dileep K Yadav, Rubina K Baithalu and Dilip K Swain

Abstract:
The present study aimed to determine the effect of trehalose and glycerol as a modified freezing diluent on the post-thaw motility of poor freezable bull semen samples at different time intervals (0 min, 30 min, 60 min, 90 min and 120 min). In the proposed study semen was collected from four Sahiwal bulls (n=6) using an artificial vagina. Split samples were used and two groups were made. Group 1 was considered as a control sample (C) in which the semen was extended with a TEYC extender containing (6.4%) and no trehalose. Group 2 was considered as a treatment sample (T) in which semen was extended in a TEYC extender containing trehalose (25%) and glycerol (5%). The treatment group underwent modified freezing whereas the control group was subjected to conventional freezing. Post-thaw incubation test was done in both groups and it was seen that the treatment sample preserved the post-thaw sperm motility of poor freezable ejaculates significantly better than the control sample at different time intervals up to 2 hrs. In conclusion, a modified freezing approach combined with trehalose and glycerol proved useful in maintaining sperm motility.