Vol. 11, Special Issue 8 (2022)

Author(s):
Nandkishor M Kanade, Ravishankar KV, Nandheesha P, Reju M Kurian and M Sankaran

Abstract:
In crosses involving polyembryonic mango varieties as a female parent the identification of resultant hybrid progenies is challenging. Therefore to confirm the hybridity, SSR markers were tried. The present study was carried out using the polyembryonic genotype, Vellaikolamban as a female parent and seven monoembryonic cultivars (Alphonso, Dashehari, Totapuri, Banganapalli, Neelum, Rumani and Amrapali) as male parents. The Vellaikolumban x Alphonso combination recorded the highest fruit set percentage, minimum days taken for germination, highest germination percentage, maximum plant height and highest rate of leaf emergence. However, seedling girth was found to be highest in Vellaikolumban x Banganapalli cross combination. The maximum fruit retention percentage was recorded in Vellaikolumban x Totapuri followed by Vellaikolumban x Alphonso and minimum in Vellaikolumban x Neelum. While, F₀ fruits of ‘Vellaikolumban x Rumani was taken maximum number of days for germination and recorded lowest germination percentage. Out of 16 SSR primers used, two markers namely MiIIHR 18 (for Vellaikolamban x Alphonso and Vellaikolamban x Totapuri cross combinations) and MiIIHR 30 (Vellaikolamban x Totapuri and Vellaikolumban x Banganapalli) gave distinct parent specific alleles.