Vol. 11, Special Issue 8 (2022)

Author(s):
R Pradeep, A Senthilkumar and A Sangaran

Abstract:
Pheasants are susceptible to a wide range of diseases, in which enteropathogens are one of the major health problems, which might be transmitted from one group of birds to other. Seventy-six fresh dropping samples were collected aseptically using readymade transport medium swabs and transported immediately to the laboratory. All the samples were processed immediately for isolation and identification of important bacterial enteropathogen Genus Salmonella using standard conventional and molecular procedures. The isolation of Genus Salmonella began with collected cultural swabs were inoculated in selenite broth and enriched culture was later streaked onto selective media xylose lysine deoxycholate agar. KB011 is a comprehensive test system that can be used for identification biochemical characterization of gram-negative Salmonella species. Molecular identification of Genus Salmonella by DNA extraction and polymerase chain reaction. Out of 76 samples, four samples were positive (05.26 Per cent) and growth of Genus Salmonella organisms on XLD appeared as pink colour colonies with blackish centers. Molecular Identification of Genus Salmonella by polymerase chain reaction the amplicon size was 260 bp.