Vol. 11, Special Issue 7 (2022)

Author(s):
Deepak Singh, DS Sahu, Gulab Chandra, Nazim Ali, Rajkumar, SP Yadav, Karunesh Kumar Dube and Kartik Tomar

Abstract:
This study aimed to explore the impact of silymarin and nano-zinc oxides on liver functions of Murrah buffalo calves. Twenty-eight Murrah buffalo calves were used in this experiment and randomly assigned into four groups (n = 7) according to body weight (136±5.16 kg) and age (10.52±0.50 months). Group 1^st acted as a control offered basal diet without any supplementation, however, group 2^nd, 3^rd, and 4^th given basal diet with silymarin (600mg/kg DM/calves/day, T₁), nano-zinc (50mg/kg DM/calves/day, T₂), and both silymarin + nano-zinc (600mg/kg DM/calves/day + 50mg/kg DM/calves/day, T₃), respectively, for 120 days of the experimental period. The jugular blood was collected at 7.00 am in EDTA containing test-tube before feeding and watering for the analysis of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP). A statistical (P< 0.05) reduction in ALT activity of the treatments was observed with respect to control, but reduction was greater in T3 groups, however, no statistical difference was noticed between T₁ and T₂ groups. The AST activity was varied statistically (P< 0.05) in the groups, and reported greater significant (P< 0.05) reduction in the activity of AST in buffalo calves who received both silymarin and nano-zinc compared to all other groups. A statistical (P< 0.05) reduction was observed in the activity of mean ALP in groups supplemented either with silymarin and nano-zinc or both than control but the reduction was highest in the T₃ group of Murrah buffalo calves. In conclusion, we find that the supplementation of silymarin and nano-zinc improved the function of liver of Murrah buffalo calves by reducing ALT, AST, and ALP.