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Vol. 11, Special Issue 7 (2022)

In vitro study on acaricidal efficacy of Azadirachta indica (Neem) methanol extracts against Rhipicephalus (B.) microplus in Udaipur (Rajasthan)

Author(s):
Vikram Punia, Hakim Manzer, Jitendra Kumar, Anita Rathore and Pravin Bano
Abstract:
Tick and tick-borne pathogens affect 80% of the world’s cattle population and are widely distributed throughout the world, particularly in the arid and semi-arid area, majorly affecting livestock production and productivity. The present research was designed to evaluate acaricidal efficacy of leaves, seeds and bark of Azadirachta indica (Neem) in methanol extracts against cattle tick Rhipicephalus (B.) microplus in Udaipur (Rajasthan). Four concentrations of the A. indica extract (10%, 25%, 50%, and 70%) and one control group with twice replications for each concentration were used in the bioassay. The Highest efficacy in both in vitro test (LPT and AIT) was recorded in methanolic extract of A. indica seeds. The A. indica seeds, leaves and bark in methanoilc extracts has highest efficacy at 70% concentration which was 84%, 63% and 50.5% respectively in LPT after 24 hrs of treatment. Minimum mortality was at 10 mg/ml (12.5%, 8% and 7.5%) respectively. In LPT, increasing mortality of larvae with increasing concentrations of Neem methanol extracts was observed. In AIT methanolic seed, leaves and bark extracts, the percentage inhibition of oviposition was 84.91%, 54.41% and 52.15% respectively at 70 mg/ml concentration. Minimum inhibition of oviposition (IO%) 40.21%, 22.62% and 9.38% in seed, leaves and bark extracts respectively at 10 mg/ml.
Pages: 731-734  |  339 Views  192 Downloads
How to cite this article:
Vikram Punia, Hakim Manzer, Jitendra Kumar, Anita Rathore and Pravin Bano. In vitro study on acaricidal efficacy of Azadirachta indica (Neem) methanol extracts against Rhipicephalus (B.) microplus in Udaipur (Rajasthan). The Pharma Innovation Journal. 2022; 11(7S): 731-734.

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