Vol. 11, Special Issue 7 (2022)

Author(s):
Ganisetti Anitha, Mukund Shiragur, Sandhyarani Nishani and Pushpa Latha G

Abstract:
Chrysanthemum (Chrysanthemum grandiflora Tzvelev) is one of the commercial crops in India. Studies were conducted for standardization of micro propagation protocol from ray florets of chrysanthemum. The experiments revealed that the highest percentage of aseptic culture and lowest percentage of contamination was observed in treatment with mercury chloride (0.1%) and ethanol (70%), the maximum callus percentage was obtained in treatment MS medium with BAP 4 mg l^-1 and NAA 1 mg l^-1, highest percentage of shoot proliferation and more number of micro shoots per clump were seen in MS medium with 5 mg l^-1 BAP and 1 mg l^-1 NAA. Number of days taken for rooting was less in half MS medium with IBA 1.5 mg l^-1. Number of roots per shoot were more in half MS medium with IBA 1.5 mg l^-1. Half MS medium with IBA 1 mg l^-1 resulted more root length in Poornima Red.