Vol. 11, Special Issue 6 (2022)

Author(s):
Veenu Singhal, Pankaj Dhakarwal, Sudesh Sharma and Taruna Bhati

Abstract:
Staphylococcus aureus is an opportunistic skin pathogen of humans and animals with clinical manifestations including skin and soft-tissue infection, sepsis and pneumonia. Haemolysin is one of the important virulence factor of S. aureus. In the present study out of 63 swabs from wound samples with pus in cattle collected from areas in and around Bikaner city, 30 S. aureus isolate were isolated with a recovery of 47.60%. All 30 samples were genotypically confirmed by 23S rRNA based ribotyping method. All the 30 isolates were typable for haemolysin genes where hla gene was detected in 29 (96.7%) and hlb gene in 25(83.3%) isolates. On sheep blood agar 22 S. aureus isolates showed haemolysis of which 11 isolates (36.7%) produced complete haemolysis, six (20%) produced incomplete haemolysis and five (16.66%) produced both types of hemolysis. Haemolysis was not shown by eight isolates. Hence S. aureus isolates obtained from open wound in cattle showed higher percentage of haemolysin both genotypically and phenotypically.