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Vol. 11, Special Issue 6 (2022)

A study on Ki67 expression in canine mammary tumors

G Thejaswini, K Aswani Kumar and P Eswara Prasad
Benign and malignant tumors of the mammary glands occur fairly frequently in female dogs that are not spayed. In fact, mammary gland tumor is the most common type of tumor diagnosed in unaltered female dogs. Canine mammary tumors have been the main focus of research for the veterinarians since past few years. Ki-67 is one of the important proliferation growth markers involved in cancer promotion and spread.
The present study was envisaged to investigate Ki-67 expression in canine mammary tissue and to correlate Ki-67 expression profile with that of different stages of metastasis in canine mammary tumors. Total RNA was isolated from twenty-six tissue samples (sixteen from tumor and ten from normal mammary tissue) using TRIzol. RNA concentration and quality were measured with a Nanodrop and by 2% agarose gel electrophoresis. First-strand cDNA was synthesized from total RNA using the Revert Aid H minus first strand cDNA synthesis kit. The generated cDNA was used as the template for quantitative real-time RT-PCR (qRT-PCR) to check the relative expression levels of Ki-67. HGPRT was used as an endogenous control. The relative gene expression levels were determined using the 2-ΔΔCT method. The qPCR data on relative expression level of Ki-67 showed up regulation of 3.52 in Canine mammary tumor. The present study showed consistent up regulation of Ki-67 in all the samples compared suggesting a significant diagnostic role of Ki-67 in canine mammary tumors.
Pages: 801-803  |  373 Views  131 Downloads
How to cite this article:
G Thejaswini, K Aswani Kumar and P Eswara Prasad. A study on Ki67 expression in canine mammary tumors. The Pharma Innovation Journal. 2022; 11(6S): 801-803.

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