Vol. 11, Special Issue 6 (2022)

Author(s):
Srinivas Rao T, Reddy KCS, Venkata Ramana K and Nagaraj P

Abstract:
The objective of this experiment was to study the effect of Catalase on the extension and preservation of canine semen. The semen parameters like per cent individual motility, per cent viability, and per cent sperm abnormalities were evaluated at refrigeration temperature (5 °C) after 0, 24, 72, and 120 hrs of collection and dilution. Six ejaculates per dog were collected by digital manipulation making a total of 36 ejaculates from six stud dogs of private owners and extended with Tris Egg Yolk Glucose Citrate (TEYGC) extender. Catalase (CAT @ 150 IU) was added to each ml of extended semen and control group (without any addition of antioxidant). The results of the present experiment reveal that the addition of catalase 150 IU improved sperm cell parameters such as per cent individual motility, per cent livability, and also decreased per cent total sperm abnormalities when compared to the control group during liquid storage of canine semen up to 120 hrs at refrigerator temperature (5 ºC). In the present study, all semen parameters significantly (P≤ 0.05) differ between test and control groups.