Flow cytometric genome size estimation of major seed spice grown in India
RS Tomar, RK Solanki, SM Padhiyar, JV Kheni and RK Kakani
Seed spices crops mainly cumin, coriander, fennel and fenugreek are predominantly cultivated in Indian sub continent to meet the domestic as well as international demand. The genomic information for genome size and genomic diversity is meagre on these crops, in the present investigation flow cytometer estimates were obtained for the 2C DNA content in term of picogram (pg) in these crops. Among the studied crops only fenugreek is from Fabaceae family rest are from the Apiaceae family; the 2C DNA content in Apiaceae crops ranged from 1.30 pg in coriander to 2.66 pg in cumin, whereas it was 7.37 pg in fenugreek. The information on genome size is useful in studying the whole genome sequence, and also to characterise the ploidy levels in the genetic resources. To our knowledge the genome size of fennel, coriander and fenugreek estimated in the present study is the first report using flow cytometry on Indian cultivars.
How to cite this article:
RS Tomar, RK Solanki, SM Padhiyar, JV Kheni and RK Kakani. Flow cytometric genome size estimation of major seed spice grown in India. The Pharma Innovation Journal. 2022; 11(6S): 507-510.