Vol. 11, Special Issue 6 (2022)

Author(s):
Garima Aggarwal, Premnath Edhigalla and Puneet Walia

Abstract:
The development of the Cetyltrimethylammonium Bromide (CTAB) protocol in plants paved the way for advances in DNA extraction.High quality plant DNA extraction is a prerequisite for molecular techniques and is crucial for crop improvement. Features of plant cell like presence of cell wall, polysaccharides and polyphenols, call for robust DNA isolation techniques. The approach utilized for extraction of DNA may vary with the age of plant sample and species. Numerous modifications have been reported for the extraction of high quality plant DNA which varies based on the sample tissue. To obtain uncontaminated high molecular weight DNA, the bulk of existing DNA extraction procedures rely on extended incubation and several precipitations or commercially available kits. Multiple methods for extracting high-quality DNA have been developed, however they are not widely used. The current review is an update on various advancements for isolating plant genomic DNA, and it encapsulates the various issues encountered and solutions devised to address them during DNA isolation from plant cells.