Vol. 11, Special Issue 6 (2022)

Author(s):
Paidipally Likhitha, JB Nayak and Sonali Thakur

Abstract:
A total of 150 carabeef samples were collected from retail meat shops in and around Anand city and isolation and identification of Staphylococcus aureus was performed. Systematic bacteriological examination resulted in the isolation of 83 Staphylococci isolates, among these 42 isolates were identified as S. aureus based on cultural, morphological, biochemical and molecular characteristics. PCR detection of nuc gene was performed simultaneously for the detection of S. aureus species. All the 42 presumptive S. aureus isolates amplified nuc gene (385 bp) and 3/42 (7.14%) of the isolates carried mecA gene (533 bp). The higher prevalence of S. aureus and MRSA indicates the lack of hygiene practices in the retail outlets in Anand and could causes several infections related to S. aureus in the consumers.