Vol. 11, Special Issue 5 (2022)

Author(s):
Dr. CM Vandana and Dr. KB Ajith Krishnan

Abstract:
The term proteomics refers to the large scale study of proteins, including their structures and functions. A proteome is much more complex than the encoding genome, and the proteins are present across a broad dynamic range as the expression of proteins are regulated in response to various external stimuli. Proteomic analysis involves separation, identification and characterization of complex mixture of proteins. The classifications of proteomics are bottom-up approach and top-down approach as well as label based and label free proteomics. Identification and characterisation of proteins or peptides are done with the help of mass spectrometer and bioinformatics tools. Animal/veterinary proteomics is an evolving field which holds a great promise not only for fundamental and applied discoveries regarding biology and pathology of domestic species, but can also be implemented in comparative applications of human diseases research. While there are certain technical limitations in the expansion of the field, they can currently be circumvented and in the future mastered with a greater participation of proteomic experts, which will in turn drive the accessibility of species-specific reagents, data volume expansion in bioinformatic databases, and increased funding.