Abstract:Nanotechnology has become one of the most important forefront fields in biology, physics and engineering. Plant-based extracts are believed to be green and effective way in the synthesis of silver nanoparticles. The present study focused on the green synthesis of silver nanoparticles using trikatu extract and evaluation of their antibacterial activity against common pathogens including
Escherichia coli and
Staphylococcus aureus.
Phytochemical analysis of the trikatu extract indicated that trikatu is a source of various phytochemicals like alkaloids, glycosides, flavonoids and diterpenes. Silver nanoparticles were prepared by mixing trikatu extract with varying concentration of silver nitrate solution.
Presence of Silver nanoparticles was confirmed by Ultaviolet-visible spectroscopy. The functional groups of the biomolecules were analysed using Attenuated total reflection spectroscopy. X- ray diffraction analysis of AgNPs indicated that the particles were crystalline in nature and were face centered cubic structure. Scanning electron microscope analysis revealed that the silver nanoparticles synthesised using trikatu were spherical shaped and the size was around 0.7 micrometer (70 nanometer).
The antibacterial potential of green synthesised silver nanoparticles was tested against the common pathogens; the Gram-negative Escherichia coli and Gram-positive Stephylococcus aureus and was shown to have good antibacterial potential against both organisms.