Vol. 11, Special Issue 5 (2022)

Author(s):
Keezia Khurshid, Syed Shariq Nazir Qadiri, Feroz Ahmad Shah, Farooz Ahmad Bhat, Bilal Ahmad Bhat, Adnan Abubakr, Shabir Ahmad Dar and Asifa Wali

Abstract:
The present study reports the establishment of a primary cell culture system from heart of Schizothorax esocinus, an indigenous cold-water fish of Indian Himalayas. The study opens a window for development of cell culture system from schizothoracids of Indian Himalayas. The standardization of the primary cell culture was done using L-15 media at different FBS concentration and temperature range. Explant method used for tissue preparation was found to be more reliable than the enzymatic dissociation technique. The primary cell culture system of heart showed consistent growth at an optimum FBS concentration of 15% and an optimum temperature of 24 ºC. Characterization of newly developed primary cell culture was done by karyotyping. Chromosome number of the primary cell culture system was found to be 98 which is consistent with the chromosome number of S. esocinus.