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Vol. 11, Special Issue 5 (2022)

Isolation, identification and antibiogram studies on P. aeruginosa and S. aureus from wound samples in and around Tirupati

Author(s):
PV Siddartha, V Kalpana, A Jagadeesh Babu, CS Swetha and T Madhava Rao
Abstract:
The present study was carried out to detect the pyogenic microorganisms from the wounds of animal origin, the antimicrobial resistant patters in and around Tirupati region. This study addresses prevalence of 2 different organisms i.e., S. aureus and P. aeruginosa by cultural and biochemical characterization from pus and wound infections and their antimicrobial resistance to different antibiotics commonly used against them. A total of 150 samples of wound and skin affections were collected from various animal species including cattle, buffalo, goats and sheep, irrespective of gender and age. Among them, 71 Staphylococcus isolates were identified and subjected to antibiotic sensitivity test using 9 different and most commonly used antibiotics. The isolates have shown maximum sensitivity towards gentamicin (83.09%) followed by co-trimaxazole (74.65%) and least sensitive to erythromycin (14.08%). Among the isolates of Staphylococcus aureus highest resistance was observed to penicillin G (78.87%) followed by erythromycin (63.38%) and least resistant to Gentamicin (9.86%). Among 150 samples, 18 samples were identified as Pseudomonas isolates and were subjected to antibiotic sensitivity test. The isolates have shown maximum sensitivity to chloramphenicol and amikacin (66.67%) and least to erythromycin (11.11%). Further these isolates have shown intermittent resistance to gentamicin (22.22%) and amikacin (11.11%). The P. aeruginosa isolates have shown maximum resistance to erythromycin (88.88%) and least resistance to gentamicin and amikacin (22.22%).
Pages: 322-329  |  397 Views  203 Downloads
How to cite this article:
PV Siddartha, V Kalpana, A Jagadeesh Babu, CS Swetha and T Madhava Rao. Isolation, identification and antibiogram studies on P. aeruginosa and S. aureus from wound samples in and around Tirupati. The Pharma Innovation Journal. 2022; 11(5S): 322-329.

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