Vol. 11, Special Issue 5 (2022)

Author(s):
Angha B Anmod and DM Jadhav

Abstract:
Citrus sinesis, citrus reticulata, Citrus aurantium and Citrus limon was most widely grown citrus species throughout the world. In this prospective study to evaluate the comparative chromatographic analysis 20 gm powdered rind of each species was extracted separately using 80% ethanol in a Soxhlet Extractor for about twelve hours. Extracts were later used for further phytochemical and HPTLC technique analysis. HPTLC chromatogram was developed in ethanolic extract of Citrus sinesis, citrus reticulata, Citrus aurantium and Citrus limon by using Toluene-Ethyl acetate-Formic acid-Methanol (8.5:1.5:0.5) as mobile phase. The study revealed that Citrus sinesis and citrus reticulata has similar chromatographic pattern showing nine phytochemical compounds. From this it can be concluded that in absence of Citrus sinesis rind one can supplement citrus reticulata rind or vice versa. Similarly Citrus limon and Citrus aurantium rind showed eight and seven polyvalent phytochemical compounds.