Vol. 11, Special Issue 5 (2022)

Author(s):
Anil Kumar, Pallav Shekhar, Bhoomika Srisant, Ajit Kumar, Anjay, Ramesh Tiwary, Anil Gattani and Ajeet Kumar

Abstract:
T. annulata is the principal causal agent of Bovine Tropical Theileriosis in India, posing a threat to animal health and productivity. The present investigation was carried out to record the molecular prevalence of Bovine Tropical Theileriosis in and around district of Patna, Bihar. A total of 200 blood samples were collected from cattle presented with clinical signs suggestive of theileriosis. The PCR based screening using the genus specific primers of 18S rRNA gene detected 120 samples (60.00%) positive for Theileria spp. The species specific primers of cytob1 gene detected 77 samples (38.50%) positive for Theileria annulata which included 12 samples found negative by Giemsa staining. Significantly, highest prevalence of disease was recorded in cattle above 2 years of age, in cross bred cattle, and in monsoon season.