Vol. 11, Special Issue 5 (2022)

Author(s):
Gawande PJ, Jumde PD and Baviskar BS

Abstract:
The immunological studies of salivary gland antigen of Rhipicephalus haemaphysaloides in rabbits was carried out in the department of Parasitology, Nagpur Veterinary College, Nagpur. Maharashtra Animal and Fishery Sciences University, Nagpur. Partially fed R. haemaphysaloides ticks were used for collection of salivary gland. The concentration of protein was estimated to be 2.1 mg/ml of antigen. Crude salivary gland antigen of R. haemaphysaloides was characterized by SDS-PAGE, which revealed 17 protein bands. It is followed by alkaline, acidic and sodium phosphate buffer treatment. Raising of hyperimmune sera was done in New Zealand white rabbits in divided doses on day 0, 14 and 28 followed by blood collection. Second booster dose was given at 2 weeks interval and final blood collection was performed 2 weeks post second booster.